Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
X-Inactivation01:58

X-Inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
X-inactivation01:58

X-inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vascular RhoJ Is an Effective and Selective Target for Tumor Angiogenesis and Vascular Disruption.

Cancer cell·2026
Same author

A Co-opted Developmental Gene Regulatory Program in Endothelial Progenitors Promotes Tumor Angiogenic Phenotypes.

Cancer research·2026
Same author

Corrigendum to Angiopoietin-2 exacerbates cardiac hypoxia and inflammation after myocardial infarction.

The Journal of clinical investigation·2026
Same author

Integrative Multiomic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors.

Cancer research·2026
Same author

Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilience.

The Journal of clinical investigation·2025
Same author

Cerebrospinal fluid draining lymphatics in health and disease: advances and controversies.

Nature cardiovascular research·2025

Related Experiment Video

Updated: Jun 2, 2026

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
12:42

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation

Published on: November 26, 2014

Taking aim at Sox18.

Injune Kim1, Gou Young Koh1,2

  • 1Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.

Elife
|February 1, 2017
PubMed
Summary
This summary is machine-generated.

A novel small molecule, Sm4, selectively inhibits the transcription factor Sox18. This targeted disruption affects Sox18 interactions without impacting other SoxF family members, offering a precise research tool.

Keywords:
biochemistrydevelopmental biologygene expressionmouseprotein-protein interactionssmall moleculesstem cellstranscription factorstumour angiogenesiszebrafish

More Related Videos

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
11:24

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 2, 2026

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
12:42

Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation

Published on: November 26, 2014

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
11:24

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Drug Discovery

Background:

  • Transcription factors regulate gene expression and are crucial in development and disease.
  • The SoxF family, including Sox18, plays significant roles in various biological processes.
  • Targeting specific transcription factors offers therapeutic potential but requires selectivity.

Purpose of the Study:

  • To identify and characterize small molecules that modulate the activity of Sox18.
  • To assess the specificity of these molecules against other SoxF family members.

Main Methods:

  • Small molecule screening assays were employed to identify inhibitors of Sox18.
  • Biochemical assays were used to confirm the inhibitory activity of Sm4.
  • Comparative analyses were performed to evaluate Sm4's impact on related SoxF proteins.

Main Results:

  • A small molecule, designated Sm4, was identified as a potent inhibitor of Sox18.
  • Sm4 effectively disrupts interactions mediated by Sox18.
  • Crucially, Sm4 demonstrated high selectivity, with minimal impact on other SoxF family members.

Conclusions:

  • Sm4 represents a selective chemical probe for studying Sox18 function.
  • This molecule has potential applications in research requiring targeted modulation of Sox18.
  • The selectivity of Sm4 provides a valuable tool for dissecting SoxF family roles.