Antioxidant defences of rabbit alveolar lining fluid

In the lower respiratory tract, alveolar cells are exposed to an oxidative challenge related to the exposure to both high levels of molecular oxygen and oxidants generated by activated phagocytes. The antioxidant defence system of alveolar cells has been thoroughly investigated, but some reports also suggest the presence of antioxidants in the layer of fluid lining the alveoli. In this report we present our studies on the antioxidant activities present in the bronchoalveolar lavage of adult rabbits. We studied total radical-trapping antioxidant capacity of surfactant and the activity of antiperoxidant enzymes. Although previous reports suggested the presence of radical scavengers, we did not find any antioxidant activity in purified surfactant. On the other hand the alveolar-lining fluid seems to contain superoxide dismutase, catalase and glutathione peroxidase, but not appreciable amounts of ferroxidase activity, as previously suggested. These enzymes could protect alveolar cells by catalyzing the dismutation of superoxide and hydrogen peroxide. The presence of glutathione peroxidase in the alveolar space seems to be physiologically relevant since the alveolar lining fluid also contains millimolar amounts of glutathione. Our studies support the concept that the alveolar lining fluid contains an active defence system against products of partial reduction of oxygen, but not chain-breaker antioxidants.