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Updated: Mar 8, 2026

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
Published on: June 7, 2019
Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity
Suvendu Biswas1, Brandon S McCullough1, Elena S Ma1
1Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, Utah 84112, USA. amy.barrios@utah.edu.
Abstract:
Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.
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