Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.8K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.8K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.8K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.8K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

2.2K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arthroscopically and manually minced cartilage demonstrates lower cell viability and lower proteoglycan deposition compared to isolated chondrons and chondrocytes.

Osteoarthritis and cartilage open·2026
Same author

SMARCD1 and Its Functional Relevance in SWI/SNF and Cancer.

International journal of molecular sciences·2026
Same author

A modified technique for mechanical isolation of stromal vascular fraction yields increased final product volume and high viable nucleated cells count.

Journal of experimental orthopaedics·2025
Same author

Key insights and implications of cartilage degradation in osteoarthritis.

Connective tissue research·2025
Same author

Identification and culture of meniscons, meniscus cells with their pericellular matrix.

Cytotherapy·2024
Same author

Transcriptomic profiling of osteoarthritis synovial macrophages reveals a tolerized phenotype compounded by a weak corticosteroid response.

Rheumatology (Oxford, England)·2024

Related Experiment Video

Updated: Mar 8, 2026

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
12:10

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: June 19, 2017

11.7K

Cellular reprogramming for clinical cartilage repair.

Britta J H Driessen1, Colin Logie2, Lucienne A Vonk3

  • 1Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.

Cell Biology and Toxicology
|February 2, 2017
PubMed
Summary
This summary is machine-generated.

Cellular reprogramming offers potential for articular cartilage repair by generating chondrocytes. Both induced pluripotent stem cell (iPSC) and direct conversion methods have limitations for clinical use, requiring further optimization.

Keywords:
Articular cartilageClinical applicationDirect lineage reprogrammingInduced pluripotent stem cellsRegenerative medicine

More Related Videos

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
08:58

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

14.8K
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
07:51

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

9.7K

Related Experiment Videos

Last Updated: Mar 8, 2026

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
12:10

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: June 19, 2017

11.7K
Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
08:58

Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model

Published on: May 21, 2013

14.8K
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
07:51

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

9.7K

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Articular cartilage repair necessitates sufficient chondrocytes, often requiring cell expansion.
  • Current cell-based therapies face limitations.
  • Cellular reprogramming presents a potential solution for generating chondrocytes.

Purpose of the Study:

  • To analyze and compare induced pluripotent stem cell (iPSC)-mediated reprogramming and direct lineage conversion for clinical cartilage repair.
  • To evaluate the qualification of derived chondrocytes for clinical application.

Main Methods:

  • Analysis of iPSC-mediated reprogramming, including small molecule/chemical compound replacement of factors.
  • Evaluation of multistage chondrogenic differentiation methods.
  • Comparison with direct fibroblast-to-chondrocyte conversion, assessing transgene requirements.

Main Results:

  • iPSC reprogramming offers insights but remains time- and cost-consuming.
  • Direct conversion is faster but requires transgene expression to prevent hypertrophy, limiting clinical translation.
  • Chondrocyte quality is highly dependent on the reprogramming method's characteristics.

Conclusions:

  • Both iPSC and direct conversion methods require further research for clinical cartilage repair.
  • Optimization necessitates proper control groups and epigenetic profiling.
  • The goal is to derive functionally stable articular chondrocytes for therapeutic use.