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Photon counting phosphorescence lifetime imaging with TimepixCam.

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Summary

A new fast optical imager, TimepixCam, uses a silicon pixel sensor for time-correlated single photon counting. This system effectively mapped phosphorescence decays in iridium complexes within polystyrene beads.

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Area of Science:

  • Photon detection
  • Optical imaging
  • Materials science

Background:

  • Fast optical imagers are crucial for advanced imaging techniques.
  • Time-correlated single photon counting (TCSPC) requires high time resolution.

Purpose of the Study:

  • To characterize a novel fast optical imager, TimepixCam.
  • To apply TimepixCam for wide-field TCSPC imaging of phosphorescence decays.

Main Methods:

  • Utilized a Timepix Application Specific Integrated Circuit (ASIC) with a 256x256 pixel silicon sensor.
  • Combined the sensor with an image intensifier for wide-field TCSPC.
  • Employed a wide-field epifluorescence microscope.

Main Results:

  • Achieved a time resolution of 15 ns at a sustained frame rate of 10 Hz.
  • Characterized photon detection capabilities of the TimepixCam system.
  • Successfully mapped phosphorescence decays of iridium complexes (approx. 1 μs lifetime) in 200 μm beads.

Conclusions:

  • TimepixCam is a capable system for wide-field TCSPC imaging.
  • The detector system demonstrates potential for analyzing short-lifetime phosphorescence in materials.