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New rapid assay for factor XIa-alpha 1 antitrypsin complex--application to DIC
Y Komiyama1, M Masuda, T Murakami
1Department of Clinico, Kansai Medical University, Osaka, Japan.
Thrombosis Research
|September 1, 1989
Summary
A new assay for factor XIa-alpha 1 antitrypsin (F.XIa-alpha 1 AT) is faster and more sensitive. Elevated F.XIa-alpha 1 AT levels in patients indicate disseminated intravascular coagulation (DIC).
Area of Science:
- Biochemistry
- Hematology
- Assay Development
Background:
- Factor XIa-alpha 1 antitrypsin (F.XIa-alpha 1 AT) complex is a marker in hemostasis.
- Previous assays for F.XIa-alpha 1 AT lacked sensitivity and speed.
Observation:
- A novel monoclonal antibody (KMXI-1) enabled a rapid F.XIa-alpha 1 AT assay.
- The new assay is completed in approximately 5 hours with a 5-fold increase in sensitivity.
- The assay is unaffected by co-existing Factor XI or plasma's nonspecific color development in diluted samples.
Findings:
- Normal F.XIa-alpha 1 AT levels in healthy adults (11 ± 4.1 ng/ml) increase with age.
- F.XIa-alpha 1 AT levels rise with disease progression in patients with disseminated intravascular coagulation (DIC).
- The onset and peak of high F.XIa-alpha 1 AT levels in DIC patients occur earlier than FDP-E or alpha 2 plasmin inhibitor-plasmin complexes.
Implications:
- F.XIa-alpha 1 AT serves as a valuable molecular marker for DIC.
- This assay offers improved diagnostic potential for coagulation disorders.
- The findings contribute to understanding F.XIa-alpha 1 AT's role in DIC pathogenesis.