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Force Measurements for Cancer Cells
Vivek Rajasekharan1,2, Varun K A Sreenivasan1,3, Brenda Farrell4
1Bobby R. Alford Department of Otolaryngology - Head & Neck Surgery, Baylor College of Medicine, Houston, TX, 77030, USA.
Abstract:
During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.
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