One-step micromolding of complex 3D microchambers for single-cell analysis
Related Experiment Video
Updated: Mar 8, 2026
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
Published on: May 24, 2019
Hiroaki Suzuki1, Kenta Mitsuno1, Katsuyuki Shiroguchi2
1Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. suzuki@mech.chuo-u.ac.jp.
Abstract:
Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.
