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Updated: Mar 8, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Evaluation of plasma oxidative stress, with or without antioxidant supplementation, in superficial partial thickness
Edoardo Raposio1, Michele P Grieco1, Edoardo Caleffi2
1a The Plastic Surgery Clinic, Department of Surgical Sciences , University of Parma , Parma , Italy.
Background:
Oxidative stress is one of the main causes of pathophysiological alterations observed during burn injury. The present pilot study aimed to determine whether a specific oral antioxidant supplementation could in any way influence free radical blood values in patients affected by superficial partial thickness burns.
Materials And Methods:
Plasma oxidants and plasma antioxidant capacity were analysed in 20 superficial partial thickness burn patients for a 2-week period; patients were randomly divided into two groups, one of which was supported with a specifically designed oral antioxidant formula (Squalene 100 mg, Vitamin C 30 mg, Coenzyme Q10 10 mg, Zinc 5 mg, Beta Carotene 3.6 mg, Bioflavonoids 30 mg, Selenium 55 mcg) administered daily, starting from the day of admission, for the whole study period.
Results:
No significant differences were found in plasma oxidants and plasma antioxidant capacity between the two groups of patients.
Conclusions:
These results did not reflect any significant benefits of an antioxidant oral supplementation at usual dosages when considering oxidative plasmatic values of superficial partial thickness burn patients.
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