COF-1-modified magnetic nanoparticles for highly selective and efficient solid-phase microextraction of paclitaxel

Area of Science:

• Materials Science
• Analytical Chemistry
• Nanotechnology

Background:

• Paclitaxel is a crucial anti-cancer drug used in chemotherapy.
• Accurate quantification of paclitaxel in biological matrices is essential for therapeutic drug monitoring.
• Existing extraction methods may lack efficiency or selectivity.

Purpose of the Study:

• To develop a novel magnetic nanoparticle-based material for paclitaxel extraction.
• To create an efficient and selective sample pretreatment method for paclitaxel analysis.
• To validate the method for application in biological samples like rat plasma.

Main Methods:

• Synthesis of covalent organic framework-1 modified magnetic nanoparticles (M-COF-1) using bio-inspired polydopamine functionalization.
• Characterization of M-COF-1 using Fourier transform infrared spectroscopy and transmission electron microscopy.
• Development and optimization of an M-COF-1 based magnetic solid-phase extraction (MSPE) method.

Main Results:

• M-COF-1 was successfully synthesized and characterized.
• The M-COF-1 MSPE method demonstrated high selectivity for paclitaxel extraction.
• The method achieved a wide linear range (0.1-200 ng/mL) and a low limit of detection (0.02 ng/mL).
• Excellent recovery rates (99.4-103.7%) and low relative standard deviations (<2.3%) were obtained for paclitaxel in rat plasma.

Conclusions:

• M-COF-1 is a promising material for the selective extraction of paclitaxel.
• The developed M-COF-1 based MSPE method provides effective enrichment and clean-up for paclitaxel in complex biological samples.
• This method enhances the accuracy and efficiency of paclitaxel analysis in clinical and research settings.