Related Experiment Video
Updated: Mar 8, 2026
Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
Published on: November 25, 2022
Application of response surface methodology for air assisted-dispersive liquid- liquid microextraction of
Mashaallah Rahmani1, Elham Ghasemi1, Mojtaba Sasani1
1Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan 98135-674, Iran.
Abstract:
A fast, simple, and easy to operate air assisted-dispersive liquid-liquid microextraction (AA-DLLME) for preconcentration and extraction of deoxynivalenol (DON) from rice samples is proposed and compared with solid phase extraction (SPE) cleanup. DON was determined using a high performance liquid chromatography with diode-array detection (HPLC-DAD). AA-DLLME was performed using a glass syringe and dispersion of extractant in the sample solution was achieved with help of air bubbles. Chloroform was used as the extractant solvent. To find out the optimized condition for the proposed method, response surface methodology (RSM) was applied for multivariate optimization of effecting parameters namely volume of extractant, number of extraction, pH, and rate of centrifugation. Under optimized condition the dynamic range of calibration graph was found to be 50-500μgL-1 with detection limit of 23.6μgL-1. Both methods were applied for extraction of DON from rice.
More Related Videos
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
10:01Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
