Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reusable interface-decoupled organic photoelectrochemical transistor biosensing for acetylcholinesterase activity and its inhibitor detection.

The Analyst·2026
Same author

Realizing Aqueous High-Order Tripartite Synapse.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Triazine-Engineered Conjugated Organic Polymers for Efficient Photocatalytic Hydrogen Peroxide Production.

ACS omega·2026
Same author

Transition metal doping boosting paper-based photoelectrochemical immunosensing for neurofilament light chain protein detection.

The Analyst·2025
Same author

Transition Metal Dichalcogenide-Based Heterojunction Band Alignment-Enabled Organic Photoelectrochemical Transistor Biosensing.

Analytical chemistry·2025
Same author

Hyperbranched Rolling Circle Amplification Enabled Nanopore Sensing Platforms for Ultrasensitive Multiplex MicroRNA Detection and Logic-Gated Molecular Diagnostics.

Analytical chemistry·2025

Related Experiment Video

Updated: Mar 8, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K

Fluorogenic boronate-based probe-lactulose complex for full-aqueous analysis of peroxynitrite.

Kai-Bin Li1, Lei Dong2, Siqi Zhang1

  • 1Department of Chemistry, Taizhou University, Jiaojiang 318000, PR China.

Talanta
|February 4, 2017
PubMed
Summary

A novel probe detects peroxynitrite (ONOO-) using a fluorogenic complex. This method enables rapid, selective analysis of ONOO- in aqueous solutions, living cells, and water samples.

Keywords:
DCMFluorescent probeFull aqueousLactulosePeroxynitrite

More Related Videos

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K

Related Experiment Videos

Last Updated: Mar 8, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K
Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

18.6K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Biology

Background:

  • Peroxynitrite (ONOO-) is a reactive nitrogen species implicated in various physiological and pathological processes.
  • Accurate detection of ONOO- is crucial for understanding its role in biological systems and environmental monitoring.
  • Existing methods for ONOO- detection can suffer from limitations in selectivity, sensitivity, or applicability in complex matrices.

Purpose of the Study:

  • To develop a selective and sensitive fluorogenic probe for the rapid analysis of peroxynitrite (ONOO-).
  • To evaluate the probe's performance in aqueous solutions, living cells, and real water samples.
  • To establish a reaction-based indicator displacement assay (RIA) for ONOO- quantification.

Main Methods:

  • Synthesis of a boronate-based fluorogenic probe by conjugating a boronic acid moiety with a DCM laser dye.
  • Characterization of the probe's fluorescence response to lactulose, forming a fluorogenic complex.
  • Investigation of the probe-lactulose complex's selective fluorescence quenching by ONOO- in the presence of other reactive oxygen and nitrogen species (ROS/RNS).
  • Application of the assay for ONOO- determination in aqueous solutions, cell imaging, and water sample analysis.

Main Results:

  • The synthesized probe exhibited an 'off-on' fluorescence response upon complexation with lactulose.
  • The probe-lactulose complex showed selective fluorescence quenching by ONOO-, with minimal interference from other tested ROS/RNS.
  • The assay demonstrated a good linear range for ONOO- quantification in aqueous media.
  • Successful monitoring of ONOO- in living cells and real water samples was achieved, highlighting the probe's practical utility.

Conclusions:

  • A novel boronate-based fluorogenic probe and lactulose complex enables rapid and selective detection of peroxynitrite (ONOO-).
  • The developed reaction-based indicator displacement assay (RIA) is suitable for quantifying ONOO- in diverse environments, including biological systems and environmental samples.
  • This approach offers a promising tool for research involving oxidative stress and for environmental monitoring applications.