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A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu2+ and its application in cells
Ying-Peng Zhang1, Yu-Ying Dong1, Yun-Shang Yang1
1School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Abstract:
A new pyrazoline-based probe D was designed and synthesized, which can be used as a highly sensitive, selective and reversible recognizing fluorescent to detect Cu2+. The recognition properties of this compound was investigated by UV-vis absorption and fluorescence spectrophotometry. The results showed that the probe D forms a 1:1 complex with Cu2+ and displayed a linear fluorescence response to Cu2+ with a detection limit of 1.94×10-7M. In addition, the probe have a good biocompatibility in living cells.

