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Multipath colourimetric assay for copper(II) ions utilizing MarR functionalized gold nanoparticles
Yulong Wang1, Limin Wang2, Zhenhe Su2
1Institute of Plant Protection, Jiangsu Academy of Agricultural Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, P.R. China.
Scientific Reports
|February 4, 2017
Summary
A new colorimetric sensor using multiple antibiotic resistance regulator (MarR) detects copper ions (Cu2+) in water. This fast, selective method offers visual, UV-vis, and smartphone detection for environmental monitoring.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Nanotechnology
Background:
- Copper ions (Cu2+) are essential but toxic at elevated levels, necessitating accurate water monitoring.
- Existing Cu2+ detection methods can be slow, expensive, or require specialized equipment.
- Developing rapid, selective, and cost-effective sensors is crucial for environmental protection.
Purpose of the Study:
- To develop a novel colorimetric sensing strategy for fast and selective detection of Cu2+ in water samples.
- To utilize multiple antibiotic resistance regulator (MarR) as a biorecognition element for Cu2+ sensing.
- To establish a multipath colorimetric assay with visual, UV-vis, and smartphone-based detection capabilities.
Main Methods:
- MarR-coated gold nanoparticles were synthesized and employed in a colorimetric assay.
- Cu2+ detection was achieved through nanoparticle aggregation induced by copper ions, causing a color change.
- The assay was validated using naked-eye observation, UV-vis spectroscopy, and smartphone imaging.
Main Results:
- The sensing system demonstrated a rapid (under 5 minutes) and selective detection of Cu2+.
- Achieved limits of detection were 1 μM (naked eye), 405 nM (UV-vis), and 61 nM (smartphone).
- The assay showed excellent performance in real water samples, confirming practical applicability.
Conclusions:
- The MarR-based gold nanoparticle assay provides a versatile and efficient platform for Cu2+ detection.
- The multipath approach enhances flexibility for on-site environmental monitoring.
- This strategy offers a novel and promising method for sensitive and selective copper ion sensing.
