Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.3K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.4K
CRISPR01:59

CRISPR

58.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.5K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

854
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
854
Homologous Recombination02:31

Homologous Recombination

64.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

Nucleic acids research·2026
Same author

Structure basis for single-strand nucleic acid targeting by IscB and variants.

bioRxiv : the preprint server for biology·2026
Same author

Conversion of IscB and Cas9 into RNA-guided RNA editors.

Cell·2025
Same author

<i>Escherichia coli</i> <i>yybP-ykoY</i> Riboswitch as a Tandem Riboswitch Regulated by Mn<sup>2+</sup> and pH.

ACS chemical biology·2025
Same author

Assessing and engineering the IscB-ωRNA system for programmed genome editing.

Nature chemical biology·2024
Same author

Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.

Molecular cell·2024

Related Experiment Video

Updated: Mar 8, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Building the Class 2 CRISPR-Cas Arsenal.

Kevin M Lewis1, Ailong Ke1

  • 1Department of Molecular Biology and Genetics, Cornell University, 251 Biotechnology Building, Ithaca, NY 14853, USA.

Molecular Cell
|February 4, 2017
PubMed
Summary

New CRISPR systems like C2c1 and C2c2 offer powerful genome-editing capabilities. Structural studies of these systems in various functional states will accelerate their application in biomedical research.

Keywords:
C2c1C2c2C2c3CRISPRCasCas9Cpf1immunityinterferencetype II

More Related Videos

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.9K
Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass
05:30

Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass

Published on: May 17, 2024

1.4K

Related Experiment Videos

Last Updated: Mar 8, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.9K
Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass
05:30

Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass

Published on: May 17, 2024

1.4K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • The CRISPR-Cas9 system has transformed genome editing.
  • Emerging single-component CRISPR effectors present new opportunities.

Purpose of the Study:

  • To characterize novel CRISPR systems, specifically C2c1 and C2c2.
  • To provide structural insights into these systems' functional states.

Main Methods:

  • Structural biology techniques (e.g., cryo-EM, X-ray crystallography).
  • Bioinformatics analysis to identify new CRISPR systems.

Main Results:

  • Generation of structural snapshots for C2c1 and C2c2.
  • Illumination of different functional states of these effector systems.

Conclusions:

  • Structural data will guide the development and application of new CRISPR tools.
  • Understanding these systems is crucial for advancing genome-editing technologies.