Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

9.2K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
9.2K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.0K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.0K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

514
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
514
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Notch Signaling Is Associated with LED Light-Regulated Papilla Regeneration in the Sea Cucumber (<i>Apostichopus japonicus</i>).

International journal of molecular sciences·2026
Same author

Acupuncture in remodeling the tumor microenvironment: current status and challenges.

Frontiers in immunology·2026
Same author

MAGNETIC INTRAOCULAR FOREIGN BODY INJURIES: Intravitreal Antibiotic Prophylaxis, Endophthalmitis Risk and Prognostic Factors.

Retina (Philadelphia, Pa.)·2026
Same author

A Biocompatible Microfluidic Sensor for Intraocular Pressure Monitoring.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Widely targeted metabolic profiling reveals drought resistance mechanisms in alfalfa leaves.

BMC plant biology·2025
Same author

HIF-1α interacting with miR-2013-3p regulates pathogen-induced energy production changes via targeting glutamate dehydrogenase in the sea urchin <i>Strongylocentrotus intermedius</i>.

Virulence·2025

Related Experiment Video

Updated: Mar 8, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

12.0K

Single-camera full-range high-resolution spectral domain optical coherence tomography.

En Bo, Si Chen, Dongyao Cui

    Applied Optics
    |February 4, 2017
    PubMed
    Summary
    This summary is machine-generated.

    We developed a novel dual-channel spectrometer for optical coherence tomography to suppress complex conjugate artifacts (CCA). This method significantly improves image quality by enhancing CCA suppression compared to previous dual-spectrometer designs.

    More Related Videos

    Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
    06:15

    Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

    Published on: August 9, 2024

    1.9K
    Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
    08:17

    Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

    Published on: September 22, 2017

    20.3K

    Related Experiment Videos

    Last Updated: Mar 8, 2026

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
    11:21

    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

    Published on: January 15, 2013

    12.0K
    Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
    06:15

    Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

    Published on: August 9, 2024

    1.9K
    Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
    08:17

    Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

    Published on: September 22, 2017

    20.3K

    Area of Science:

    • Biomedical Optics
    • Optical Imaging
    • Ophthalmic Imaging

    Background:

    • Complex conjugate artifacts (CCA) and direct current (DC) artifacts degrade image quality in spectral domain optical coherence tomography (SD-OCT).
    • Previous dual-spectrometer methods for CCA suppression suffer from channel mismatch, limiting performance.

    Purpose of the Study:

    • To develop and validate a novel dual-channel spectrometer for enhanced complex conjugate artifact (CCA) suppression in SD-OCT.
    • To achieve full-range imaging with improved CCA and DC artifact reduction.

    Main Methods:

    • A dual-channel spectrometer utilizing a three-line charge-coupled device (CCD) was employed to capture two spectra with a 2π/3 phase difference.
    • Complex interferometric signals were reconstructed using trigonometric manipulation, enabling full-range image retrieval via inverse Fourier transform.
    • The dual-channel design uses identical spectrometer optics to minimize spectral channel mismatch, improving CCA suppression.

    Main Results:

    • The novel dual-channel spectrometer achieved CCA suppression up to approximately 25 dB in imaging an attenuated reflector.
    • Effective suppression of both CCA and DC artifacts was demonstrated in ex vivo rat eye anterior segment imaging.
    • Image quality in CCA-suppressed images was significantly superior to that obtained with previous dual-spectrometer designs.

    Conclusions:

    • The developed dual-channel spectrometer offers superior performance in CCA suppression compared to existing dual-spectrometer solutions.
    • This technology significantly enhances image quality in SD-OCT by mitigating critical artifacts.
    • The system provides a robust platform for high-quality full-range imaging in biomedical applications.