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RIPK1 binds to vitamin D receptor and decreases vitamin D-induced growth suppression

Waise Quarni1, Panida Lungchukiet1, Anfernee Tse1

  • 1The Departments of Pathology and Cell Biology, University of South Florida College of Medicine, Tampa, FL 33612, United States.

The Journal of Steroid Biochemistry and Molecular Biology
|February 5, 2017
PubMed

Insights

Receptor interacting protein kinase 1 (RIPK1) negatively regulates vitamin D signaling by inhibiting the vitamin D receptor (VDR). Depleting RIPK1 enhances vitamin D

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Receptor interacting protein kinase 1 (RIPK1) is implicated in cell survival and death pathways.
  • RIPK1 is known to confer drug resistance in cancer cells.
  • The role of RIPK1 in vitamin D signaling remains unexplored.

Purpose of the Study:

  • To investigate the role of RIPK1 in 1,25-dihydroxyvitamin D3 (1,25D3)-induced growth suppression.
  • To determine if RIPK1 modulates the activity of the vitamin D receptor (VDR).

Main Methods:

  • Luciferase reporter assays to assess VDR transcriptional activity.
  • Co-immunoprecipitation to detect RIPK1-VDR complex formation.
  • Subcellular fractionation to analyze VDR localization.
  • Experiments using RIPK1-null MEFs and RIPK1-knockdown cancer cells.

Main Results:

  • RIPK1 inhibited VDR transcriptional activity independently of its kinase activity.
  • RIPK1 directly binds to the VDR ligand-binding domain.
  • RIPK1 promotes VDR retention in the cytoplasm, reducing its transcriptional function.
  • 1,25D3-induced growth suppression was enhanced in RIPK1-deficient cells.

Conclusions:

  • RIPK1 acts as a repressor of VDR.
  • RIPK1 depletion potentiates 1,25D3-mediated growth suppression.
  • Targeting RIPK1 could enhance vitamin D-based cancer therapies.
Keywords:
ApoptosisCancerNecroptosisNuclear receptorReceptor interacting protein kinase 1Vitamin DVitamin D receptor

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