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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Mar 8, 2026

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Live-cell Imaging of Single-Cell Arrays LISCA - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Accelerating Live Single-Cell Signalling Studies.

Sam Cooper¹, Chris Bakal²

¹The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK; Department of Computational Systems Medicine, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.

Trends in Biotechnology

|February 6, 2017

Summary

This summary is machine-generated.

Live single-cell imaging now allows detailed characterization of signalling networks. Advances in fluorescent sensors and data analysis enable high-throughput studies of cellular responses to environmental changes.

Failed At:

2026-06-19T13:37:40.555080+00:00

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Last Updated: Mar 8, 2026

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