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Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control
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A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
Owen W Duckworth1, Dawn S Markarian1, Dorothy L Parker2
1Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695, USA.
Journal of Microbiological Methods
|February 6, 2017
Abstract:
Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.
