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Related Experiment Video

Updated: Mar 8, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
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Lipid nanoparticles assessment by flow cytometry.

Anna Bryła1, Wojciech Juzwa2, Marek Weiss3

  • 1Institute of Chemical Technology and Engineering, Poznan University of Technology, 4 Berdychowo Street, 60-965 Poznan, Poland.

International Journal of Pharmaceutics
|February 6, 2017
PubMed
Summary

A novel flow cytometry method quantifies liposomes, measuring size and structure simultaneously. This high-throughput technique offers a fast and reliable alternative for liposome assessment in their native environment.

Keywords:
Drug carriersDrug deliveryFlow cytometryLiposomesNanocarriers

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Analytical Chemistry

Background:

  • Liposomes are crucial drug delivery carriers, with size and structure being key parameters.
  • Existing methods struggle to simultaneously assess liposome size and structure at high throughput.
  • A novel flow cytometry method has been developed for liposome quantification.

Purpose of the Study:

  • To develop and validate a novel flow cytometry method for simultaneous liposome size and structure assessment.
  • To compare the efficacy of different fluorescent staining combinations for liposome analysis.
  • To evaluate the performance of the developed method against established techniques like DLS and AFM.

Main Methods:

  • Tested fluorescent staining combinations: DiD/TO, Rh123/DiD, Syto9/DiD.
  • Compared three liposome populations (raw, E10, E21) using flow cytometry.
  • Utilized Dynamic Light Scattering (DLS) for average diameter and size distribution.
  • Employed Atomic Force Microscopy (AFM) for structural analysis.

Main Results:

  • The DiD/TO fluorochrome combination effectively resolved liposome sub-populations.
  • DLS analysis identified three distinct vesicle populations with mean diameters of 323nm, 220nm, and 170nm.
  • AFM confirmed structural differences between raw and extruded liposomes.
  • Flow cytometry results showed good agreement with DLS and AFM data.

Conclusions:

  • Flow cytometry, with appropriate fluorescent dyes, is a suitable method for liposome assessment.
  • The developed method provides fast, reliable, and simultaneous analysis of liposome size and structure.
  • This technique enables liposome analysis in their native environment, overcoming limitations of current methods.