Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities
Kamila Goderska1, Wojciech Juzwa2, Tomasz M Karpiński3
1Department of Food Technology of Plant Origin, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland.
Molecules (Basel, Switzerland)
|November 27, 2024
Summary
This study optimized lactobionic acid production using Pseudomonas taetrolens bacteria, achieving a high yield of 37.42 g/L. The research also explored the acid's prebiotic and antagonistic properties, with successful microencapsulation of Bifidobacterium bifidum.
Area of Science:
- Biotechnology
- Microbiology
- Biochemistry
Background:
- Whey, a byproduct of the dairy industry, presents an opportunity for sustainable bioprocessing.
- Lactobionic acid (LBA) is a valuable compound with potential applications in food and pharmaceutical industries.
- Optimizing bacterial fermentation for LBA production is crucial for its commercial viability.
Purpose of the Study:
- To quantitatively analyze lactobionic acid (LBA) production by Pseudomonas taetrolens using whey.
- To identify and evaluate key culture parameters influencing LBA yield.
- To assess the prebiotic and antagonistic properties of LBA.
Main Methods:
- Bioreactor cultivation of Pseudomonas taetrolens with whey as a medium.
- Optimization of culture parameters, including lactose concentration and bacterial strain selection.
- Quantification of lactose and LBA using high-performance liquid chromatography (HPLC).
- Flow cytometry with imaging for analyzing microbial metabolic activity.
- In vitro gastrointestinal model to evaluate microencapsulated Bifidobacteria survival and prebiotic effects.
Main Results:
- The highest LBA yield achieved was 37.42 g/L under optimized conditions (2% lactose, specific Pseudomonas taetrolens strain).
- Flow cytometry provided insights into microbial metabolic activity during LBA fermentation.
- Microencapsulation of Bifidobacterium bifidum with LBA demonstrated enhanced survival in a simulated gastrointestinal environment.
- Lactobionic acid exhibited antagonistic activity, suggesting potential antimicrobial applications.
Conclusions:
- Pseudomonas taetrolens is an effective microorganism for LBA production from whey.
- Optimized bioreactor conditions significantly enhance LBA yield.
- Lactobionic acid possesses promising prebiotic properties and demonstrates antagonistic activity.
Keywords:
BifidobacteriumPseudomonas taetrolensflow cytometryin vitro modellactobionic acidlactoseprebioticwheyMore Related Videos
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