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Updated: Mar 7, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Heme-binding activity of methoxyflavones from Pentzia monodiana Maire (Asteraceae)
Sergio Ortiz1, Kamel Dali-Yahia2, Pedro Vasquez-Ocmin3
1Laboratoire de Pharmacognosie, UMR CNRS 8638 COMETE, Faculté de Pharmacie de Paris, Université Paris-Descartes, Sorbonne Paris Cité, 4, av. de l'Observatoire, 75006 Paris, France.
Abstract:
A heme-binding assay based on mass spectrometry was performed on P. monodiana Maire (Asteraceae) extracts to identify metabolites able to form adducts with heminic part of haemoglobin, as potential antimalarial drugs. Main adducts were characterized and their stability was measured. Isolation of main constituents of P. monodiana Maire lead to identification of the two methoxyflavones 3'-O-methyleupatorin (7) and artemetin (8) involved in the adducts formation. Four seco-tanapartholides (1-4), a guaianolide (5), a germacranolide (6) and two other methoxyflavones (9, 10) were also characterized. Evaluation of isolated compounds on P. falciparum and T. brucei brucei showed a moderate antiprotozoal activity of the two methoxyflavones.
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