A stretchable and screen-printed electrochemical sensor for glucose determination in human perspiration

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Accurate glucose monitoring is crucial for diabetes management.
• Existing methods can be invasive or expensive.
• Development of low-cost, wearable glucose sensors is needed.

Purpose of the Study:

• To develop and evaluate printable, stretchable, and inexpensive glucose biosensors.
• To assess the performance of enzymatic biosensors for glucose determination in physiological fluids.
• To explore the potential for non-invasive, on-body glucose monitoring.

Main Methods:

• Fabrication of platinum (Pt)-decorated graphite-based sensors.
• Enzymatic biosensor utilizing glucose oxidase immobilized on Pt-decorated graphite.
• Chronoamperometric detection of hydrogen peroxide (H2O2) reduction at -0.35V (vs pseudo-Ag/AgCl).
• Testing in phosphate buffer solution (0.25M PBS, pH 7.0) and real human perspiration samples.

Main Results:

• The enzymatic biosensor demonstrated excellent performance in phosphate buffer solution with a linear range of 0 mM to 0.9 mM.
• High sensitivity, selectivity, and a low limit of detection (LOD) were achieved.
• Successful application in real human perspiration samples.
• Significant correlation observed between perspiration glucose concentration and blood glucose levels measured by a commercial meter.

Conclusions:

• Printable, stretchable, and inexpensive Pt-decorated graphite biosensors offer a viable alternative for glucose determination.
• The developed enzymatic biosensor shows promise for non-invasive, on-body glucose monitoring.
• This technology could provide a convenient and cost-effective tool for diabetes management.