An optimized transurethral catheterization cystometry in mice and comparison with classic suprapubic catheterization...

Area of Science:

• Urology
• Animal Models
• Physiology

Background:

• Accurate urodynamic assessment in mice is crucial for understanding bladder dysfunction.
• Existing methods for cystometry and urethral pressure profiling in mice can be invasive or complex.

Purpose of the Study:

• To establish an optimized, minimally invasive transurethral catheterization cystometry (TUCC) for mice.
• To develop a novel urethral pressure profile (UPP) measurement technique for mice.
• To validate these methods in a partial bladder outlet obstruction (pBOO) model.

Main Methods:

• Optimized TUCC and UPP measurement techniques were developed and established.
• TUCC was compared with suprapubic catheterization cystometry (SCC) in anesthetized female mice post-suprapubic catheter implantation (SCI).
• The optimized TUCC and UPP were applied to a pBOO mouse model to record urodynamic parameters.

Main Results:

• TUCC demonstrated significant changes in micturition volume, residual urine, bladder capacity, and micturition pressure after SCI.
• No significant differences were observed in key urodynamic parameters between TUCC and SCC after SCI.
• A significant increase in maximum urethral pressure was observed in the pBOO model.

Conclusions:

• The minimally invasive TUCC and UPP measurement provide a feasible, repeatable, and accessible method for studying mouse bladder function.
• These optimized techniques are suitable for investigating urodynamic changes in mouse models, including those with induced bladder outlet obstruction.