Related Experiment Videos
[Identification of HLA B27 antigen by flow cytometry]
1Service d'Immunologie cellulaire, Centre Régional de Transfusion Sanguine, Bordeaux.
Annales De Biologie Clinique
|January 1, 1989
Summary
Flow cytometry using immunofluorescence aids in identifying cell surface antigens like Human Leukocyte Antigen B27 (HLA B27). This method effectively distinguishes individuals with the HLA B27 phenotype, often associated with rheumatic diseases.
Area of Science:
- Immunology
- Cell Biology
- Medical Diagnostics
Context:
- Cell surface antigen identification is crucial for understanding disease mechanisms.
- Human Leukocyte Antigen B27 (HLA B27) is a well-established marker associated with rheumatic diseases.
- Accurate phenotyping of HLA B27 is essential for clinical diagnosis and research.
Purpose:
- To describe the application of cytofluorograph analysis with immunofluorescence for Human Leukocyte Antigen B27 (HLA B27) identification.
- To demonstrate the utility of monoclonal antibodies in detecting specific cell surface antigens.
- To establish a method for differentiating HLA B27 positive and negative individuals.
Summary:
- Cytofluorograph analysis, utilizing immunofluorescence and monoclonal antibodies, enables precise identification of cell surface antigens.
- This technique was specifically applied to detect the Human Leukocyte Antigen B27 (HLA B27) on cell membranes.
- Fluorescence histograms generated by the analysis clearly differentiate subjects possessing the HLA B27 phenotype from those who are HLA cross-linked.
Impact:
- Provides a reliable method for HLA B27 phenotyping, aiding in the diagnosis and study of associated rheumatic diseases.
- Enhances the capability of cytofluorometry in immunological and clinical applications.
- Facilitates clearer differentiation between individuals with and without the HLA B27 antigen, improving disease association studies.