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Related Experiment Video

Updated: Mar 7, 2026

Translating Ribosome Affinity Purification TRAP for RNA Isolation from Endothelial Cells In Vivo

Translating Ribosome Affinity Purification TRAP for RNA Isolation from Endothelial Cells In Vivo

Published on: May 25, 2019

Rap1 in endothelial biology.

Magdalena Chrzanowska-Wodnicka¹

¹Blood Research Institute, BloodCenter of Wisconsin, Part of Versiti, Milwaukee, Wisconsin.

Current Opinion in Hematology

|February 9, 2017

Summary

The small GTPase Rap1 is crucial for stabilizing blood vessels and maintaining endothelial function, particularly nitric oxide release. Understanding Rap1

Area of Science:

Endothelial biology
Molecular signaling
Vascular physiology

Background:

Small GTPase Rap1 regulates integrin and cadherin processes.
Rap1 is downstream from cAMP-activated Epac in endothelium.
Rap1 influences angiogenesis and endothelial barrier function.

Purpose of the Study:

To investigate the physiological role of Rap1 in mouse models.
To uncover molecular details of endothelial barrier regulation.
To understand Rap1's role in vessel development and adult conditions.

Main Methods:

In vivo studies using mouse models.
Genetic manipulation (double knockout of Rap1 isoforms).
Analysis of endothelial barrier function, nitric oxide production, and cell adhesion.

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Last Updated: Mar 7, 2026

Translating Ribosome Affinity Purification TRAP for RNA Isolation from Endothelial Cells In Vivo

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Published on: May 25, 2019

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Published on: August 20, 2019

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

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Published on: May 5, 2023

Main Results:

Rap1 is essential for vessel stabilization, not initial formation.
Rap1 knockout leads to hemorrhage and embryonic lethality.
Rap1 is critical for nitric oxide production and endothelial function post-development.
Radil and Afadin mediate Rap1's effects on the endothelial barrier.

Conclusions:

Rap1 is vital for nitric oxide release and endothelial function in vivo.
Rap1 is a key regulator of endothelial cell shear stress responses and homeostasis.
Understanding Rap1 mechanisms may reveal therapeutic targets for vascular diseases.