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Low molecular weight polyethylenimine-conjugated gold nanospheres: a platform for selective gene therapy controlled
Fei Liu1,2, Fen-Fen Kong2, Qing-Po Li2
1Department of Radiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou City 310016, Zhejiang Province, People's Republic of China.
Aim:
Whether PEI2k-HAuNS could promote gene transfection efficiency controlled by near-infrared (NIR) light.
Materials & Methods:
This safe nonviral gene delivery system was obtained by conjugating low molecular weight (2 kDa) polyethylenimine (PEI) onto hollow gold nanospheres (PEI2k-HAuNS). Upon NIR laser irradiation, there was a conspicuous increase both in the in vitro and in vivo transfection achieved by the nanocomplexes. Furthermore, a plasmid encoding the tumor suppressor TP53 (pTP53) was applied to test antitumor activity.
Results:
The enhanced gene transfection efficiency and therapy of PEI2k-HAuNS were achieved via the mediation of an NIR laser compared with the other treatments in vitro and in vivo.
Conclusion:
The application of NIR laser irradiated PEI2k-HAuNS can be used as a promising gene delivery systems in vitro and in vivo.
