Related Experiment Video
Updated: Mar 7, 2026
Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal
Published on: January 26, 2019
CD24 Modulates Chemosensitivity of MCF-7 Breast Cancer Cells
Hideya Onishi1, Kumi Suyama2, Akio Yamasaki2
1Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan ohnishi@surg1.med.kyushu-u.ac.jp.
Abstract:
The role of cluster of differentiation (CD) 24 in breast cancer remains unclear; previously, we showed that CD24 suppresses malignant phenotypes by inactivating Hedgehog signaling through signal transducer and activator of transcription (STAT) 1 inhibition. In this study, we examined how CD24 affects chemosensitivity in breast cancer cells. The CD44+CD24+ breast cancer cell line MCF-7 was transfected with CD24 with/without STAT1 siRNA, and chemosensitivity to 5-fluorouracil (5-FU) and cis-diamminedichloroplatinum (CDDP) was measured. CD24 inhibition reduced chemosensitivity to 5-FU, while STAT1 inhibition did not affect chemosensitivity to 5-FU in CD24 siRNA-transfected cells. Conversely, CD24 inhibition did not affect chemosensitivity to CDDP, while STAT1 inhibition reduced chemosensitivity to CDDP in CD24 siRNA-transfected cells. STAT1 inhibition, but not CD24 inhibition, reduced expression of the ATP-binding cassette (ABC) transporter genes, ABCB1 and ABCG2. In conclusion, CD24 inhibition may modulate chemosensitivity according to drug type, but ABC transporter expression appears not to contribute to this mechanism. This study contributes to determining the role of CD24 in breast cancer.
Insights
Cluster of differentiation (CD) 24 affects breast cancer chemosensitivity differently based on the drug. CD24 inhibition impacts 5-fluorouracil sensitivity, while signal transducer and activator of transcription (STAT) 1 inhibition affects cis-diamminedichloroplatinum sensitivity.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- The role of cluster of differentiation (CD) 24 in breast cancer pathogenesis is not fully understood.
- Previous research indicated CD24 suppresses malignant phenotypes via Hedgehog signaling and signal transducer and activator of transcription (STAT) 1 inhibition.
Purpose of the Study:
- To investigate the effect of CD24 on the chemosensitivity of breast cancer cells.
- To elucidate the mechanisms underlying CD24's influence on drug response.
Main Methods:
- MCF-7 breast cancer cells (CD44+CD24+) were transfected with CD24 and/or STAT1 siRNA.
- Chemosensitivity to 5-fluorouracil (5-FU) and cis-diamminedichloroplatinum (CDDP) was assessed.
- Expression of ATP-binding cassette (ABC) transporter genes (ABCB1, ABCG2) was analyzed.
Main Results:
- CD24 inhibition reduced chemosensitivity to 5-FU, but not CDDP.
- STAT1 inhibition reduced chemosensitivity to CDDP, but not 5-FU.
- STAT1 inhibition, unlike CD24 inhibition, decreased ABCB1 and ABCG2 gene expression.
Conclusions:
- CD24's modulation of breast cancer chemosensitivity is drug-dependent.
- The observed effects do not appear to be mediated by alterations in ABC transporter gene expression.
- This study provides insights into CD24's complex role in breast cancer treatment response.
