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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Related Experiment Video

Updated: Mar 7, 2026

Polysome Profiling without Gradient Makers or Fractionation Systems

Polysome Profiling without Gradient Makers or Fractionation Systems

Published on: June 1, 2021

Analysis of translation using polysome profiling.

Héloïse Chassé^1,2, Sandrine Boulben^1,2, Vlad Costache^1,2

¹CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff cedex, France.

Nucleic Acids Research

|February 10, 2017

Summary

This summary is machine-generated.

This study optimizes polysome profiling for analyzing translational regulation in gene expression. The improved method aids in studying gene regulatory networks, especially in new model organisms.

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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Translational regulation of gene expression is increasingly recognized for its importance across many organisms.
Polysome profiling is a key technique for assessing mRNA translational status and analyzing the translatome (actively translated mRNAs).
This method is particularly valuable for novel model systems with limited genomic data.

Purpose of the Study:

To describe an optimized protocol for sea urchin polysome purification.
To demonstrate the application of this protocol in studying mRNA translational regulation post-fertilization.
To provide a method for high-throughput processing of polysome fractions for large-scale sequencing.

Main Methods:

Optimized polysome purification protocol for sea urchins.
Application of the protocol to analyze mRNA translational changes after fertilization.
Development of high-throughput processing for polysome gradient fractions.

Main Results:

An optimized and critical-step-highlighted protocol for sea urchin polysome purification was established.
The protocol was successfully applied to experimentally investigate translational regulation of mRNAs following fertilization.
A method for high-throughput processing of polysome gradient fractions was demonstrated.

Conclusions:

The developed polysome profiling protocol is effective for studying translational regulation in sea urchins.
This protocol can be integrated into gene regulatory network studies in diverse biological models.
The high-throughput processing capability facilitates large-scale sample preparation for next-generation sequencing.