Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

9.1K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.1K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

5.4K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Online Detection and Rejection Method for Consecutive Outliers in Underwater Long-Baseline Positioning Based on Kinematic Constraints.

Sensors (Basel, Switzerland)·2026
Same author

Full-Spectrum-Activated Confining Aerogel With Dual-Scale Photothermal Regulation for Ultra-Efficient Low-Temperature Water Purification.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Effects of intermittent fasting combined with resistance training on training adaptations: an exploratory multilevel meta-analysis.

Frontiers in nutrition·2026
Same author

[Advances in the application of the HEART score in patients with acute coronary syndromes].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences·2026
Same author

Pan-cancer analysis of tissue-plasma genomic concordance reveals enhanced concordance with liver metastasis and plasma clonality as predictor of poorer overall survival.

NPJ precision oncology·2026
Same author

Evaluation of Interfacial Adhesion Properties Between Nano-Modified Composite Polyurethane and Cement-Stabilized Macadam Based on Surface Free Energy.

Materials (Basel, Switzerland)·2026

Related Experiment Video

Updated: Mar 7, 2026

Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

11.5K

Apoptotic Bodies: Selective Detection in Extracellular Vesicles.

Paul Hauser1, Sha Wang1, Vladimir V Didenko2

  • 1Baylor College of Medicine, and Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX, 77030, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2017
PubMed
Summary

Researchers developed a new fluorescence technique to specifically identify apoptotic bodies among extracellular vesicles. This method targets DNA with specific breaks, aiding apoptosis research involving microvesicles and exosomes.

Keywords:
5′P blunt-ended DNA breaksApoptotic bodiesApoptotic bodies labelingCell signaling in apoptosisClearance of apoptotic cellsExosomesExtracellular vesiclesIn situ ligation techniqueMicrovesicles“eat-me” signal“find-me” signal

More Related Videos

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

13.6K
Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

9.2K

Related Experiment Videos

Last Updated: Mar 7, 2026

Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

11.5K
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
06:15

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

13.6K
Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

9.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cells release various membrane-bound vesicles like microvesicles, exosomes, and apoptotic bodies, crucial for intercellular communication.
  • Current purification methods struggle to differentiate between these extracellular vesicle subtypes due to variations in size and properties.
  • Distinguishing apoptotic bodies is vital for understanding apoptosis and its role in cellular processes.

Purpose of the Study:

  • To develop a specific fluorescence-based method for identifying apoptotic bodies within mixed extracellular vesicle preparations.
  • To enable precise discrimination between apoptotic bodies and other extracellular vesicles like microvesicles and exosomes.
  • To provide a tool for enhanced studies of apoptosis involving extracellular vesicles.

Main Methods:

  • A novel fluorescence technique was developed to specifically label extracellular vesicles.
  • The technique targets DNA fragments with 5'PO4 blunt-ended DNA breaks, characteristic of apoptotic DNA degradation.
  • This method allows for the selective identification of apoptotic bodies in heterogeneous vesicle samples.

Main Results:

  • The fluorescence technique successfully and specifically identifies apoptotic bodies.
  • It distinguishes apoptotic bodies from microvesicles and exosomes in mixed preparations.
  • The method relies on the presence of specific DNA breaks generated during apoptosis.

Conclusions:

  • This fluorescence technique offers a reliable way to specifically identify apoptotic bodies.
  • It overcomes limitations of current purification methods in discriminating extracellular vesicle subtypes.
  • The technique is a valuable tool for research on apoptosis and extracellular vesicle biology.