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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment.

Jessica Kalra^1,2,3, Jennifer Baker⁴

¹Department of Experimental Therapeutics, British Columbia Cancer Agency, 675 West 10th Avenue, 5th Floor, Vancouver, BC, V5Z 1L3, Canada. jessica.kalra@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|February 11, 2017

Summary

This summary is machine-generated.

Multiplex Immunohistochemistry (mIHC) enables simultaneous detection of multiple tissue markers in one section. This technique is crucial for understanding complex biological processes and drug mechanisms.

Keywords:

Immunofluorescence In vivo co-localization Molecular expression in vivo Multiplex immunohistochemistry (mIHC)Pathology Quantitative image analysis Target validation Tissue microarrays Tumor microenvironment

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Last Updated: Mar 7, 2026

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

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Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Area of Science:

Biomedical research
Molecular pathology
Cancer research

Background:

Multiplex Immunohistochemistry (mIHC) allows simultaneous detection of multiple targets in a single tissue section.
Enables evaluation of signal transduction pathways, marker co-localization, and molecular distribution within tissues.
Crucial for characterizing molecular signaling and drug action mechanisms.

Purpose of the Study:

To describe the principles of designing and implementing multiplex immunohistochemistry (mIHC) protocols.
To create parameter tumor maps for various labeled markers.
To facilitate detailed analysis of tissue microenvironments and molecular interactions.

Main Methods:

Utilizes multiplex immunohistochemistry (mIHC) for simultaneous multi-target detection.
Involves designing and implementing mIHC protocols.
Employs automated slide imaging and analysis for medium to high-throughput data acquisition.

Main Results:

Demonstrates the capability of mIHC to analyze tumor microenvironments, including vascular architecture and hypoxia.
Shows utility in assessing cellular proliferation, cell death, and drug distribution.
Enables the creation of parameter tumor maps for multiple markers.

Conclusions:

Multiplex Immunohistochemistry (mIHC) is a powerful technique for comprehensive tissue analysis.
The described protocol facilitates detailed mapping of molecular markers in various biological contexts.
mIHC supports advanced research in cancer biology and drug development.