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Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley, Nepal
Eiji Haramoto1, Masaaki Kitajima2
1Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan. eharamoto@yamanashi.ac.jp.
Abstract:
Aichi virus 1 genomes were detected by quantitative PCR in groundwater from shallow dug (10/22) and tube wells (1/15), river water (14/14), and sewage (1/1), with the maximum concentration of 4.0 × 109 copies/l. Nucleotide sequencing analysis demonstrated the prevalence of genotype B in the virus positive samples.
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