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Updated: Mar 7, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

A Robust Protocol for Protein Extraction and Digestion.

Michelle Atallah¹, Mark R Flory¹, Parag Mallick²

¹Canary Center at Stanford for Cancer Early Detection, Stanford University, 3155 Porter Drive, Palo Alto, CA, 94304, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 12, 2017

Summary

This summary is machine-generated.

Standardized proteomics protocols ensure consistent protein analysis. This study details optimized methods for protein extraction, digestion, and cleanup for reliable mass spectrometry data in biological research.

Keywords:

Acetone precipitation Enzymatic solution digest Liquid chromatography-mass spectrometry Protein extraction Proteomics

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Last Updated: Mar 7, 2026

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Published on: February 27, 2020

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

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Published on: February 7, 2022

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Published on: July 19, 2019

Area of Science:

Biochemistry and Molecular Biology
Cellular Biology
Analytical Chemistry

Background:

Proteins are crucial for cellular homeostasis and biological processes.
Proteomics offers deep insights into cellular functions and disease mechanisms.
Sample preparation variability impacts proteomic data reliability.

Purpose of the Study:

To present standardized protocols for protein extraction and preparation for mass spectrometry.
To enhance consistency and integrity in proteomic sample handling.
To address the critical need for standardization in proteomic workflows.

Main Methods:

Optimized protein extraction from cell cultures and tissues.
In-solution enzymatic digestion of proteins into peptides.
Peptide cleanup and preparation for liquid chromatography-mass spectrometry (LC-MS).

Main Results:

Developed and validated reproducible protein preparation methods.
Ensured maximum consistency in sample handling and analysis.
Maintained sample integrity throughout the proteomic workflow.

Conclusions:

Standardized protocols are essential for reliable and reproducible proteomic studies.
These methods facilitate accurate investigation of biological and disease processes.
Consistent sample preparation is paramount for high-quality mass spectrometry data.