Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

13.5K
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
13.5K
Thermosensation01:43

Thermosensation

34.2K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
34.2K
Olfaction01:25

Olfaction

49.3K
The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
49.3K
Physiology of Smell and Olfactory Pathway01:20

Physiology of Smell and Olfactory Pathway

13.5K
Humans detect odors with the help of specialized cells located in the upper part of the nasal cavity, called olfactory receptor neurons (ORNs). ORNs possess hair-like structures called cilia, which are receptive to sensations from the inhaled air. When an odorant molecule binds to a specific receptor on the cell of the cilia, it leads to a series of events that ultimately cause the ORN to send electrical signals to the olfactory bulb in the brain through the olfactory nerves.
The olfactory...
13.5K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

6.4K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GDF15 (Growth/Differentiation Factor-15) Expression in Human Adipose Tissue and in Adipocyte Cell Lines.

Biomedicines·2026
Same author

Obesity- and Glucose-Dependent Differential Autophagy Marker Expression in Adipose Tissues and Adipocytes.

Obesity science & practice·2026
Same author

Oral losartan yields subtherapeutic airway exposure compared to nebulized delivery and fails to improve mucociliary clearance in cystic fibrosis.

Frontiers in pharmacology·2026
Same author

In-situ product recovery in microfluidic bioreactors.

Current opinion in biotechnology·2026
Same author

Establishment of a Single-Cell Minimal Medium: A Case Study for Microfluidic Cultivation of Corynebacterium glutamicum.

Biotechnology and bioengineering·2026
Same author

Age-Dependent Systemic Regulation of C1q/TNF-Related Protein 3 and Progranulin in Patients with Cystic Fibrosis: Biomarkers or Therapeutic Targets?

Biomedicines·2026
Same journal

Reflections from Mathias Bähr, PhD, Editor-in-Chief for Molecular and Cellular Neuroscience, 2017-2026.

Molecular and cellular neurosciences·2026
Same journal

Advancing molecular and cellular neuroscience: Vision and priorities from the new editor-in-chief.

Molecular and cellular neurosciences·2026
Same journal

Apolipoprotein E in Alzheimer's disease: A review of APOE receptors, signalling pathways and therapeutic opportunities.

Molecular and cellular neurosciences·2026
Same journal

Inadvertent p75NTR signaling might cause inconsistencies in the neuroprotection offered by mesenchymal stem cells.

Molecular and cellular neurosciences·2026
Same journal

Corrigendum to "Depressed mitochondrial function and electron transport Complex II-mediated H2O2 production in the cortex of type 1 diabetic rodents" [Mol. Cell. Neurosci. Volume 90, July 2018, Pages 49-59].

Molecular and cellular neurosciences·2026
Same journal

Sleep deprivation and hippocampal integrity: Oxidative stress mediated neuronal, memory and behavioral alterations and the restorative role of sleep recovery.

Molecular and cellular neurosciences·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
15:40

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

Published on: August 10, 2013

38.2K

Trpm5 expression in the olfactory epithelium.

Martina Pyrski1, Eugenia Eckstein1, Andreas Schmid1

  • 1Center for Integrative Physiology and Molecular Medicine, Saarland University, Homburg, Germany.

Molecular and Cellular Neurosciences
|February 12, 2017
PubMed
Summary
This summary is machine-generated.

Transient Receptor Potential Melastatin 5 (Trpm5) channels are not found in adult mouse olfactory sensory neurons (OSNs). A novel Trpm5 splice variant, Trpm5-9, is expressed in OSNs, with Trpm5 transiently present during embryonic development.

Keywords:
Odor detectionOlfactory systemPheromonal sensingTRP channel

More Related Videos

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
10:16

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

27.5K
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

15.8K

Related Experiment Videos

Last Updated: Mar 7, 2026

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization
15:40

An Effective Manual Deboning Method To Prepare Intact Mouse Nasal Tissue With Preserved Anatomical Organization

Published on: August 10, 2013

38.2K
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
10:16

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

27.5K
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

15.8K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Sensory Biology

Background:

  • Transient Receptor Potential Melastatin 5 (Trpm5) is crucial for taste chemotransduction.
  • Its presence and function in olfactory sensory neurons (OSNs) of the main olfactory epithelium (MOE) remain debated.
  • Specifically, its role in pheromonal detection systems in adult mice requires clarification.

Purpose of the Study:

  • To investigate the expression and potential function of Trpm5 channels in adult mouse OSNs.
  • To determine if Trpm5 is involved in specific olfactory detection systems, such as pheromonal detection.
  • To characterize any novel Trpm5 splice variants or expression patterns in the olfactory system.

Main Methods:

  • Utilized a novel Trpm5-IRES-Cre knockin mouse model for genetic labeling.
  • Employed validated Trpm5 antibodies for protein detection.
  • Conducted in situ hybridization to localize Trpm5 RNA.
  • Searched for Trpm5 splice variants in genetically labeled MOE cells.
  • Performed Ca2+ imaging using GCaMP3 in a newly developed MOE wholemount preparation.

Main Results:

  • Found no evidence of the classical Trpm5 channel in adult mouse OSNs.
  • Identified a novel splice variant, Trpm5-9, in Trpm5-expressing adult OSNs, which is unlikely to function independently.
  • Demonstrated transient Trpm5 expression in a subpopulation of mature OSNs during embryonic development.
  • Revealed that Trpm5-expressing OSNs in adult mice are heterogeneous and can detect general odorants.

Conclusions:

  • The classical Trpm5 channel is absent in adult mouse olfactory sensory neurons.
  • A novel Trpm5 splice variant (Trpm5-9) is present but likely non-functional alone.
  • Trpm5 may play a transient role during embryonic olfactory development.
  • Trpm5-expressing OSNs in adults are diverse and respond to general odorants, contributing to our understanding of olfactory transduction.