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Updated: Mar 7, 2026
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Highly selective optical and reversible dual-path chemosensor for cyanide detection and its application in live cells
Karunesh Keshav1, Pritam Torawane2, Mukesh Kumar Kumawat3
1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076 India.
Abstract:
A new biocompatible fluorescent receptor 1 was synthesized by conjugating diaminomaleonitrile (DMN) with benzothiazole unit, and characterized by single X-ray. In DMF/water (1:1, v/v), the receptor 1 showed a selectivity turn-on fluorescence at 517nm in the presence of CN-. Receptor 1 showed a detection limit down to 0.16µM without any interference from other tested anions. The reversibility and reusability of 1 for the detection of CN- ion was also tested for five cycles indicating the probe 1 could be used in a reversible manner. Importantly, the receptor 1 showed excellent cells viability and was successfully applied for the detection of CN- in live mouse fibroblast cells L929 cells.
