Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Record Carrier Diffusion Lengths in Large, Dense Nonfullerene Electron Acceptor Crystals Grown from Polar Aromatic Solvents.

Journal of the American Chemical Society·2026
Same author

S-palmitoylation of the v-ATPase subunit RNAseK is necessary for Zika virus infection.

Virology journal·2026
Same author

Redesigning EWOD Interconnections: Inkjet-Printed PEDOT:PSS Electrodes with Enhanced Pad Access.

ACS omega·2026
Same author

GBP1 recruitment to actin-rich pedestals of extracellular Gram-negative bacteria promotes pyroptosis.

The EMBO journal·2026
Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026
Same author

Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress.

Nature communications·2026
Same journal

Reliability of A Vibration-Based Elastography Protocol For Assessing Achilles Tendon Stiffness Across Multiple Joint Angles In Elite Athletes.

Journal of visualized experiments : JoVE·2026
Same journal

Associations of Inflammatory and Coagulation Biomarkers with Kidney Injury Across Chronic and Acute Clinical Settings.

Journal of visualized experiments : JoVE·2026
Same journal

Intelligent Recommender Systems for Chinese Super League Fan Consumption Behavior Prediction.

Journal of visualized experiments : JoVE·2026
Same journal

A Battery of Quantitative Binocular Vision Tests for Adults: Testing Protocols.

Journal of visualized experiments : JoVE·2026
Same journal

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026
Same journal

Clinical Efficacy of Tissue-Bone Homeostasis Manipulation on Soft Tissue Balance and Function in Knee Osteoarthritis.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Mar 7, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.6K

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy.

Frederik Görlitz1, Douglas J Kelly2, Sean C Warren2

  • 1Photonics Group, Department of Physics, Imperial College London; f.gorlitz14@imperial.ac.uk.

Journal of Visualized Experiments : Jove
|February 13, 2017
PubMed
Summary
This summary is machine-generated.

We developed an open-source, automated instrument for high-content analysis using fluorescence lifetime imaging (FLIM) to study protein interactions via Förster resonance energy transfer (FRET). This system enables screening of cell signaling and identification of binding partners in multiwell plates.

More Related Videos

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

10.2K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

26.0K

Related Experiment Videos

Last Updated: Mar 7, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.6K
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

10.2K
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

26.0K

Area of Science:

  • Biophysics
  • Cell Biology
  • Biotechnology

Background:

  • Protein interactions are crucial for cellular functions.
  • Förster resonance energy transfer (FRET) is a powerful technique for studying molecular interactions.
  • High-content screening of protein interactions in cells is essential for drug discovery and understanding cellular processes.

Purpose of the Study:

  • To present an open-source, automated high-content analysis instrument for fluorescence lifetime imaging (FLIM).
  • To enable assaying protein interactions using FRET in fixed or live cells within multiwell plates.
  • To facilitate screening of cell signaling and identification of protein binding partners.

Main Methods:

  • Automated multiwell plate fluorescence lifetime imaging (FLIM).
  • Utilizing Förster resonance energy transfer (FRET) for protein interaction and biosensor studies.
  • Open-source data acquisition software (µManager) and analysis tools (FLIMfit for OMERO).

Main Results:

  • Demonstrated functionality of the automated multiwell plate FLIM instrument.
  • Presented exemplar data on HIV Gag protein oligomerization.
  • Showcased a time-course study of a FRET biosensor in live cells.

Conclusions:

  • The developed instrument provides a versatile platform for high-content screening of protein interactions using FLIM-FRET.
  • Open-source software and hardware facilitate practical implementation and data analysis.
  • This approach is applicable to studying both intramolecular FRET biosensors and intermolecular protein interactions.