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Updated: Mar 7, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
A Label-free and Turn-on Fluorescence Strategy for Kanamycin Detection Based on the NMM/G-quadruplex Structure
Hualin Yang1, Qinghua Wu, Dongxiao Su
1Jingchu Food Research and Development Center, College of Life Science, Yangtze University.
Abstract:
In the present work, a simple design of a "label-free" fluorescence method for kanamycin was developed and explored based on a specific DNA aptamer as a recognizer and a NMM/G-quadruplex DNA system as a reporter. The titration experiment showed a linear detection range of 0.5 to 100 nM with a low detection limit of 0.5 nM. It also showed excellent selectivity in a selectivity experiment. It was then successfully employed to detect kanamycin in milk with a excellent reliability.
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