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Genome-Wide Analysis of the Circulating miRNome After Cerebral Ischemia Reveals a Reperfusion-Induced MicroRNA
Stefan Uhlmann1, Eva Mracsko1, Ehsan Javidi1
1From the Department of Neurology, University Heidelberg, Germany (S.U., E.M., E.J., R.V.); High-Throughput Genomics Group at the Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom (S.L., A.T.); Genomics Proteomics Core Facility HUSAR Bioinformatics Lab, German Cancer Research Center (DKFZ), Heidelberg, Germany (A.H.-W., K.-H.G.); Division of Brain Sciences, Imperial College London, United Kingdom (R.V.).
Background And Purpose:
Circulating microRNAs (miRNAs) are emerging biomarkers for stroke because of their high stability in the bloodstream and association with pathophysiologic conditions. However, the circulating whole-genome miRNAs (miRNome) has not been characterized comprehensively in the acute phase of stroke.
Methods:
We profiled the circulating miRNome in mouse models of acute ischemic and hemorrhagic stroke by next-generation sequencing. Stroke models were compared with sham-operated and naive mice to identify deregulated circulating miRNAs. Top-ranked miRNAs were validated and further characterized by quantitative reverse transcription polymerase chain reaction.
Results:
We discovered 24 circulating miRNAs with an altered abundance in the circulation 3 hours after ischemia, whereas the circulating miRNome was not altered after intracerebral hemorrhage compared with sham-operated mice. Among the upregulated miRNAs in ischemia, the top-listed miR-1264/1298/448 cluster was strongly dependent on reperfusion in different ischemia models. A time course experiment revealed that the miR-1264/1298/448 cluster peaked in the circulation around 3 hours after reperfusion and gradually decreased thereafter.
Conclusions:
Alteration of the miRNome in the circulation is associated with cerebral ischemia/reperfusion, but not hemorrhage, suggesting a potential to serve as biomarkers for reperfusion in the acute phase. The pathophysiological role of reperfusion-inducible miR-1264/1298/448 cluster, which is located on chromosome X within the introns of the serotonin receptor HTR2C, requires further investigation.
