Related Experiment Video

Updated: Mar 7, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.3K

Proteomics reveals a new DNA repair factor involved in DNA damage signaling

Markus Räschle1

  • 1Department of Molecular Genetics, TU Kaiserslautern , Kaiserslautern, Germany.

Molecular & Cellular Oncology
|February 16, 2017
PubMed

Insights

A new DNA replication activator, ETAA1, is essential for maintaining genome stability during replication stress. Its malfunction may be linked to pancreatic cancer development.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • DNA replication stress activates the ATR checkpoint kinase.
  • ATR coordinates cellular responses to replication stress.
  • The precise mechanisms of ATR activation are under investigation.

Purpose of the Study:

  • To identify novel activators of the ATR checkpoint kinase.
  • To elucidate the role of ETAA1 in DNA replication and genome maintenance.
  • To explore the potential involvement of ETAA1 in pancreatic cancer.

Main Methods:

  • Utilized molecular biology techniques to study protein interactions.
  • Investigated the function of ETAA1 in cellular models of replication stress.
  • Analyzed ETAA1 expression and its correlation with pancreatic cancer.

Main Results:

  • Identified ETAA1 as a novel and essential activator of ATR.
  • Demonstrated that ETAA1 is crucial for maintaining genome integrity under replication stress.
  • Observed dysregulation of ETAA1 in pancreatic cancer tissues.

Conclusions:

  • ETAA1 plays a critical role in the DNA replication checkpoint.
  • ETAA1 is indispensable for preventing genomic instability.
  • ETAA1 dysregulation represents a potential driver for pancreatic cancer.
Keywords:
ATR signalingDNA Replication checkpointDNA repairProteomics

More Related Videos

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.7K
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.8K

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.5K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
41.4K
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
34.5K
Overview of DNA Repair02:25

Overview of DNA Repair

10.2K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K