Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vesicular stomatitis virus P function depends on cellular growth cycle.

C P Stanners, S Kennedy, L Poliquin

    Virology
    |September 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What is Comprehensive Geriatric Assessment (CGA)? An umbrella review.

    Age and ageing·2017
    Same author

    Outcomes of telemedicine intervention in a regional intensive care unit: a before and after study.

    Anaesthesia and intensive care·2017
    Same author

    Metastatic melanoma: prognostic factors and survival in patients with brain metastases.

    Journal of neuro-oncology·2017
    Same author

    Summarizing US Wildlife Trade with an Eye Toward Assessing the Risk of Infectious Disease Introduction.

    EcoHealth·2017
    Same author

    120 Years of Tuberculosis Research: An Historical Perspective from the Archive of the Journal of Comparative Pathology.

    Journal of comparative pathology·2017
    Same author

    International estimated fetal weight standards of the INTERGROWTH-21<sup>st</sup> Project.

    Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2016
    Same journal

    Viral diversity in predatory insects used for biological control: Insights from a multi-country meta-viromic analysis.

    Virology·2026
    Same journal

    Unveiling the hidden virome of Sclerotinia sclerotiorum: New tools and discoveries in mycovirus detection.

    Virology·2026
    Same journal

    ASFV pDP238L negatively regulates type I interferon production via inhibiting the methylation of TBK1.

    Virology·2026
    Same journal

    Divergent poxvirus identified in a non-native black rat from Madagascar.

    Virology·2026
    Same journal

    Genomic and functional characterization of Bacillus phage BCE1 targeting a key gut bacterium in Aedes albopictus larvae.

    Virology·2026
    Same journal

    Viral etiology of orogenital papillomatosis and squamous cell carcinoma in bottlenose dolphins in the southeastern United States.

    Virology·2026
    See all related articles

    Vesicular stomatitis virus (VSV) P function, which reduces protein synthesis, is dependent on the cell

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • The P function of vesicular stomatitis virus (VSV) is characterized by reduced total protein synthesis in infected cells due to decreased translational machinery efficiency.
    • The existence and detectability of VSV P function have been debated, with conflicting results between laboratories regarding wild-type VSV (HR) and a P- mutant (T1026-R1).

    Purpose of the Study:

    • To investigate the discrepancy in VSV P function detection between different laboratories.
    • To elucidate the factors influencing the manifestation of VSV P function in infected cells.

    Main Methods:

    • Infection of L-cell cultures with VSV strains (HR, T1026-R1, tsG31) at different growth phases (exponential, stationary).
    • Analysis of total protein synthesis rates in infected cells.

    Related Experiment Videos

  • Characterization of the P function phenotype (P+, P-, P++) based on protein synthesis reduction.

    • Main Results:

    • VSV P function is dependent on the growth phase of infected L-cell cultures; it is less apparent in early exponential phase and more pronounced in stationary phase.
    • The VSV P- mutant T1026-R1 consistently lacks P function across all growth phases.
    • A VSV M protein mutant, tsG31, exhibits a P++ phenotype, causing a greater reduction in protein synthesis than wild-type HR, and displays P function even in early exponential phase cells.

    Conclusions:

    • The detectability of VSV P function is significantly influenced by the cellular growth phase.
    • VSV P function is not universally absent in early exponential phase cells, contrary to previous findings.
    • The M protein plays a role in the P function phenotype, with the tsG31 mutant demonstrating a distinct and potent effect on protein synthesis.