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Related Experiment Videos

Enterovirus replication in porcine ileal explants.

B A Heinz1, D O Cliver, B Donohoe

  • 1Food Research Institute, University of Wisconsin, Madison 53706.

The Journal of General Virology
|September 1, 1987
PubMed
Summary

Porcine ileum explants maintained cellular structure in specific media. Coxsackievirus B5 replicated best in young pig absorptive tissue, but overall viral yields were low.

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Area of Science:

  • Veterinary Virology
  • Gastroenterology
  • Cell Biology

Background:

  • Porcine ileum explants are used to study viral infections.
  • Understanding viral replication in swine tissues is crucial for disease control.

Purpose of the Study:

  • To evaluate tissue preservation of porcine ileum explants in culture.
  • To assess the replication efficiency of coxsackievirus B5 in different porcine ileum tissues.

Main Methods:

  • Porcine ileum explants were cultured in CMRL-1066 medium with insulin and cortisone.
  • Tissue preservation was assessed using light and electron microscopy.
  • Explants were infected with coxsackievirus B5 or human poliovirus type 1, and progeny virus was quantified via plaque assay.

Main Results:

  • Cellular structure of ileum explants was well-maintained for up to 48 hours in supplemented CMRL-1066.
  • Coxsackievirus B5 replicated most efficiently in absorptive tissue explants from young pigs.
  • Viral replication occurred in both absorptive and lymphoid tissues of adult pigs, but overall infection was inefficient with low progeny virus yield.

Conclusions:

  • CMRL-1066 supplemented with insulin and cortisone supports porcine ileum explant viability for up to 48 hours.
  • Porcine ileum explants can be used to study coxsackievirus B5 replication, with variations observed based on tissue type and animal age.
  • The study highlights the inefficiency of coxsackievirus B5 infection in these explant models, suggesting limitations for high-yield virus production.

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