Reactivation with Pancreatin of Coproantibody-Neutralized Virus in Ground Beef

Viruses transmissible through food are sometimes shed in association with coproantibody, which may preclude their detection by conventional means. We had shown previously that a large volume of food extract (up to 1.4 ml/cm² of monolayer) can be tested in cell culture, and that the inoculum can be transferred from one type of cell culture to another to afford a broader spectrum of virus sensitivity. Here we report that pancreatin is capable of reactivating the majority of coproantibody-neutralized poliovirus and that pancreatin-treated extract from ground beef samples can be tested in large volumes and transferred among cell cultures as described above.