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Related Experiment Video

Updated: Mar 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Collagen-Gold Nanoparticle Conjugates for Versatile Biosensing.

Sarah Unser¹, Samuel Holcomb², ReJeana Cary³

¹Department of Chemistry, College of Arts and Sciences, University of Cincinnati, 301 West Clifton Court, Cincinnati, OH 45221-0172, USA. unsersa@mail.uc.edu.

Sensors (Basel, Switzerland)

|February 18, 2017

Summary

Researchers developed a novel biosensor by combining collagen with gold nanoparticles. This innovative sensor can detect both glucose and heparin colorimetrically, showing potential for versatile diagnostic applications.

Keywords:

collagen gold nanoparticles localized surface plasmon resonance optical biosensors plasmonic biosensing plasmonic coupling

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Last Updated: Mar 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

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Published on: March 7, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

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Published on: June 23, 2016

Area of Science:

Biomaterials Science
Nanotechnology
Analytical Chemistry

Background:

Noble metal nanoparticles integrated with proteins offer potential for versatile biosensors.
Proteins provide selectivity, while nanoparticles offer unique optical properties for colorimetric detection.

Purpose of the Study:

To develop a protein-nanoparticle conjugate for colorimetric biosensing.
To utilize collagen's extracellular matrix interactions for selective analyte detection.

Main Methods:

Integration of collagen with 10 nm gold nanoparticles to form a conjugate.
Detection of glucose via collagen fibril cross-linking and nanoparticle proximity (LSPR shift).
Detection of heparin using a competition assay with heparin-gold nanoparticles.

Main Results:

The collagen-nanoparticle conjugate selectively detects both glucose and heparin.
Detection occurs within the physiological concentration range for both analytes.
Glucose detection in 50% mouse serum shows a linear range of 3-25 mM.

Conclusions:

Collagen-gold nanoparticle conjugates offer a versatile platform for colorimetric biosensing.
The developed biosensor demonstrates potential for detecting clinically relevant analytes.
This approach enables simple, selective, and colorimetric detection of glucose and heparin.