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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
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Engineering approaches in siRNA delivery.

Anna Angela Barba1, Sara Cascone2, Diego Caccavo2

  • 1Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II, 132, 84084, Fisciano (SA), Italy.

International Journal of Pharmaceutics
|February 19, 2017
PubMed
Summary
This summary is machine-generated.

Short interfering RNAs (siRNAs) show great therapeutic potential but face delivery challenges. Mathematical modeling offers a promising framework to optimize siRNA delivery systems and overcome these limitations for future clinical applications.

Keywords:
Delivery vectorsMathematical modelingPhysical modelingin vitro modelssiRNAs

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Area of Science:

  • Biotechnology
  • Pharmacology
  • Biophysics

Background:

  • Short interfering RNAs (siRNAs) are potent gene silencers with significant therapeutic potential, especially for inflammatory diseases.
  • Clinical application of siRNAs is hindered by poor stability, rapid clearance, and inefficient cellular uptake.
  • These limitations necessitate advanced strategies for effective siRNA delivery and therapeutic use.

Purpose of the Study:

  • To review mathematical modeling approaches for siRNA delivery systems.
  • To analyze the production and release of siRNA vectors from hydrogels.
  • To explore the modeling of siRNA pharmacokinetics and biodistribution.

Main Methods:

  • Analysis of mathematical modeling techniques for siRNA delivery.
  • Review of siRNA vector production and hydrogel release processes.
  • Examination of physical models for siRNA fate in biological systems.

Main Results:

  • Mathematical models can elucidate phenomena in siRNA dosage system preparation and drug-body interactions.
  • Modeling aids in improving the design of siRNA delivery systems, processes, and therapies.
  • Physical models offer insights into siRNA vector behavior in bloodstream and tissues.

Conclusions:

  • Mathematical and physical modeling provide a framework for understanding and optimizing siRNA therapeutics.
  • While not yet fully realized, these modeling approaches show promising potential for future clinical applications of siRNAs.
  • Continued development in modeling can address current limitations and enhance the therapeutic efficacy of siRNA-based treatments.