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A spectrophotometric screening method for avermectin oxidizing microorganisms.

Yuan-Shan Wang¹, Qi-Wei Hu¹, Xing-Chang Zheng¹

¹Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China; Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang Province 310014, PR China.

Journal of Microbiological Methods

|February 21, 2017

Summary

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This summary is machine-generated.

A new spectrophotometric method was developed to screen for microbes that oxidize avermectin. This technique successfully identified microorganisms capable of regioselectively converting avermectin to 4″-oxo-avermectin.

Area of Science:

Microbiology
Biotechnology
Analytical Chemistry

Background:

Avermectin is a potent antiparasitic drug.
Microbial oxidation offers a potential route for avermectin derivatization.
Efficient screening methods are needed to identify relevant microorganisms.

Purpose of the Study:

To establish a spectrophotometric screening method for avermectin-oxidizing microbes.
To identify microorganisms that regioselectively oxidize avermectin to 4″-oxo-avermectin.

Main Methods:

Development of a spectrophotometric assay based on the reaction of 4″-oxo-avermectin with 2,4-dinitrophenylhydrazine.
Utilizing a gradient High-Performance Liquid Chromatography (HPLC) assay for confirmation.

Main Results:

Keywords:

2,4-Dinitrophenylhydrazine 4″-Oxo-avermectin Avermectin Regioseletive oxidation

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A reliable spectrophotometric screening method for avermectin oxidizing microbes was established.
Microorganisms capable of regioselective oxidation of avermectin to 4″-oxo-avermectin were successfully obtained.

Conclusions:

The developed spectrophotometric method, coupled with HPLC, is effective for isolating avermectin-oxidizing microorganisms.
This method facilitates the discovery of biocatalysts for avermectin modification.