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Updated: Mar 7, 2026
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Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples
Published on: May 21, 2021
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Human eosinophils constitutively express a unique serine protease, PRSS33
Sumika Toyama1, Naoko Okada2, Akio Matsuda2
1Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan; Department of Immune Regulation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Summary
Eosinophils produce protease serine 33 (PRSS33), which is expressed on their surface. This PRSS33 may drive fibroblast extracellular matrix production, contributing to airway remodeling in asthma.
Area of Science:
- Immunology
- Cell Biology
- Respiratory Medicine
Background:
- Eosinophils are key players in asthma pathogenesis, particularly airway remodeling.
- Their role in producing proteases, crucial for tissue modification, remains incompletely understood.
Purpose of the Study:
- To investigate eosinophil-specific protease production using transcriptome analysis.
- To elucidate the role of eosinophil proteases in airway remodeling.
Main Methods:
- Purification of human eosinophils and other cells from peripheral blood.
- Evaluation of protease expression and release using transcriptomic, proteomic, and cellular assays.
- Assessment of fibroblast response to recombinant protease serine 33 (PRSS33).
Main Results:
- Eosinophils express high levels of eosinophil-specific protease serine 33 (PRSS33) mRNA.
- PRSS33 is localized to eosinophil granules and increases on the cell surface upon stimulation.
- Recombinant PRSS33 stimulates fibroblast collagen and fibronectin mRNA expression.
Conclusions:
- Eosinophil cell surface PRSS33 may induce fibroblast extracellular matrix synthesis.
- This mechanism potentially explains eosinophils' contribution to airway remodeling in asthma.
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