Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

1.7K
Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the...
1.7K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

2.4K
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
2.4K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.6K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.6K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.6K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing Liquid Vortex Capture & the Rapid Droplet Sampling Interface for Single Cell Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

One Step Block-like Copolymers from an Upcycled Monomer Using Ring-Opening Metathesis Polymerization.

ACS polymers Au·2026
Same author

Rapid Generation of Tandem Mass Spectrometry Reference Libraries Using Immediate Drop-on-Demand Liquid Handling Coupled to an Open Port Sampling Interface.

Journal of the American Society for Mass Spectrometry·2026
Same author

Correction: HunStat2 - a simple and low-cost potentiostat with electrochemical impedance spectroscopy capability.

Analytical methods : advancing methods and applications·2026
Same author

HunStat2 - a simple and low-cost potentiostat with electrochemical impedance spectroscopy capability.

Analytical methods : advancing methods and applications·2025
Same author

Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: Mar 7, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.8K

Atomic Force Microscopy Thermally-Assisted Microsampling with Atmospheric Pressure Temperature Ramped Thermal

William D Hoffmann1, Vilmos Kertesz1, Bernadeta R Srijanto2

  • 1Mass Spectrometry and Laser Spectroscopy Group, Chemical Sciences Division, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831, United States.

Analytical Chemistry
|February 21, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a novel atomic force microscopy method for analyzing polymer thin films. The technique enables reproducible, spatially resolved sampling and mass spectrometric analysis of polymer oligomers.

More Related Videos

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

10.1K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K

Related Experiment Videos

Last Updated: Mar 7, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09:22

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

14.8K
Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

10.1K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Polymer Science

Background:

  • Characterizing polymer thin films requires precise sampling techniques.
  • Existing methods may lack spatial resolution or be limited in molecular weight range.

Purpose of the Study:

  • To introduce and validate a nanothermal analysis probe method for spatially resolved sampling of polymer thin films.
  • To assess the reproducibility, sampling size, and oligomer distribution analysis capabilities.
  • To explore the method's potential for identifying individual oligomers and its limitations.

Main Methods:

  • Utilized atomic force microscopy (AFM) with controlled nanothermal analysis probes for thermally assisted sampling.
  • Sampled micrometer-sized areas of poly(2-vinylpyridine) (P2VP) thin films.
  • Analyzed sampled materials using atmospheric pressure chemical ionization and mass spectrometry (MS).

Main Results:

  • Demonstrated reproducible sampling of P2VP thin films with number-average molecular weights (Mn) below 3000.
  • Mass spectrometry data for lower Mn P2VP correlated well with supplier values.
  • Higher Mn P2VP samples showed intact oligomers and thermolysis products; individual oligomers were identified via tandem MS.

Conclusions:

  • The nanothermal analysis probe method offers a viable approach for spatially resolved oligomer analysis of polymer thin films.
  • The technique's effectiveness is dependent on polymer molecular weight, with higher Mn samples yielding thermolysis products.
  • Future improvements could enhance spatial resolution and extend the temperature range for analysis.