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Related Experiment Video

Updated: Mar 7, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

Thermoresponsive polymer-modified microfibers for cell separations.

Kenichi Nagase¹, Yoichi Sakurada², Satoru Onizuka¹

¹Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.

Acta Biomaterialia

|February 22, 2017

Summary

Thermoresponsive polymer-modified microfibers enable temperature-controlled cell separation. These materials selectively adhere specific cells, facilitating the isolation of fibroblasts and adipose-derived stem cells from complex mixtures.

Keywords:

Cell separation Electrospun microfiber Polymer brush Regenerative medicine Stimuli responsive polymer

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Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

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Last Updated: Mar 7, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

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Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Area of Science:

Biomaterials Engineering
Polymer Science
Cell Biology

Background:

Developing advanced materials for cell separation is crucial in regenerative medicine and research.
Thermoresponsive polymers offer tunable properties for dynamic biological applications.
Microfibers provide a high surface area scaffold for cell interactions.

Purpose of the Study:

To create thermoresponsive polymer-modified microfibers for temperature-controlled cell separation.
To investigate the selective adhesion of different cell types to these modified microfibers.
To assess the potential of these materials for isolating specific cell populations, such as stem cells.

Main Methods:

Electrospinning of poly(4-vinylbenzyl chloride) (PVBC) to create microfibers.
Surface-initiated atom transfer radical polymerization (ATRP) to graft poly(N-isopropylacrylamide) (PIPAAm).
Optimization of electrospinning conditions and PIPAAm grafting for controlled fiber properties.

Main Results:

Successfully prepared PVBC microfibers modified with PIPAAm exhibiting thermoresponsive behavior.
Demonstrated thermally controlled selective adhesion and detachment of normal human dermal fibroblasts and adipose-derived stem cells (ADSCs).
Achieved successful separation of ADSCs from mixed adipose tissue-derived cells by temperature modulation.

Conclusions:

PIPAAm-modified microfibers function as effective temperature-modulated cell separation materials.
The unique fiber structure enhances cell adhesion compared to flat substrates.
These materials hold potential for applications in cell sorting and regenerative medicine.