Therapeutic Drug Monitoring Using a Poly(N-isopropylacrylamide) Hydrogel-Modified Bead-Packed Column with an...

Area of Science:

• Analytical Chemistry
• Materials Science
• Biomedical Engineering

Background:

• Therapeutic drug monitoring (TDM) is crucial for optimizing drug efficacy and safety.
• Current TDM methods often rely on organic solvents and extensive sample preparation.
• A need exists for simpler, safer, and more efficient TDM techniques.

Purpose of the Study:

• To develop and evaluate a novel chromatography column for TDM.
• To enable TDM using an aqueous mobile phase and temperature-sensitive materials.
• To eliminate the need for organic solvents and complex sample preparation in TDM.

Main Methods:

• Silica beads were modified with poly(N-isopropylacrylamide) (PNIPAAm) hydrogels via condensation and radical polymerization.
• A chromatography column was packed with the modified silica beads.
• Drug elution behavior was studied using an aqueous mobile phase at varying temperatures.
• Separation of model serum samples containing drugs and proteins was performed.
• Drug concentrations were quantified using calibration curves.

Main Results:

• The PNIPAAm-modified silica bead column demonstrated temperature sensitivity.
• Successful separation of serum proteins and model drugs was achieved at 30°C using an aqueous mobile phase.
• The column enabled drug concentration quantification without organic solvents.
• The method showed potential for simplified sample preparation.

Conclusions:

• The engineered chromatography column offers a promising advancement for therapeutic drug monitoring.
• This temperature-sensitive system provides a safe, straightforward, and solvent-free approach to TDM.
• The developed method simplifies TDM by reducing reliance on organic solvents and complex sample preparation.