Anisotropic Mesenchymal Stem Cell Sheet Composed of Adipose-Tissue- and Umbilical-Cord-Derived Mesenchymal Stem Cells

Mesenchymal stem cell (MSC) sheet therapies are effective in treating intractable diseases. Aligned and oriented bone-marrow-derived MSC sheets have been developed using stripe-patterned thermoresponsive cell culture dishes to increase the effectiveness of MSC therapies compared with unaligned MSC sheets. However, adipose-tissue- and umbilical-cord-derived MSCs (ADMSCs and UCMSCs, respectively) have not yet been used for preparing aligned cell sheets. Therefore, we prepared aligned cell sheets comprising ADMSCs and UCMSCs. We produced a patterned cell culture dish by modifying polyacrylamide into a striped pattern on a commercially available poly(N-isopropylacrylamide)-modified dish. Aligned cell sheets comprising ADMSCs and UCMSCs were successfully prepared in these culture dishes.